Skip to main content

Table 2 Primers used in this study

From: Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. coliĀ from inexpensive aromatic precursors

Primers

Sequence (5ā€² to 3ā€²)

ltaEP.p -F

AGGAGAAAGGTACCATGACAGACAAGAGCCAACAATTCGCCAGCG

ltaEP.p-R

AGGTCGACATAGTTAATTTCTCCTACTAGTTCAGCCACCAATGATCGTG

A8HB.t-F

TGGCTGAACTAGTAGGAGAAATTAACTATGTCGACCTCACCCCACCGCCCCGCTCATC

A8HB.t-R

ATAGTTTTGCTCATAGTTAATTTCTCCTGCTAGCTCTAGATCACGGCCACGACATG

ltaEE.c-F

TTAAAGAGGAGAAAGGTACCATGATTGATTTACGCAGTGATACCG

ltaEE.c-R

GGTGAGGTCGACATAGTTAATTTCTCCTACTAGTTTAACGCGCCAGGAATGCACGCC

ltaEC.c-F

AAGAGGAGAAAGGTACCTTGATGACCCAGACCGCGCCCCGCTACG

ltaEC.c-R

GTGAGGTCGACATAGTTAATTTCTCCTCTAAGCCACTCGCTTCAGCGCCGCGCCCAGA

ilvAB.a-F

AACTAGTAGGAGAAATTAACTATGTCGACTGAACAACAGGGCACCGCCCA

ilvAB.a-R

GTTTTATTTGATGCCTCTAGATCAGTGCTGCAGTTTCGCGTG

CGCGA

TAAB.t-F

GTGGCTGAACTAGTAGGAGAAATTAACTATGTCTGCTCAACCCGCCTCCGACCTCG

TAAB.tT-R

GTTTTATTTGATGCCTCTAGATCACCCGCGCAGGAACTCGCCGTAGCGCGCG

ridA-F

TCGTGGCCGTGAGCTAGCAGGAGAAATTAACTATGAGCAAAACTATCGCGA

ridA-R

CCTTTCGTTTTATTTGATGCCTCTAGATTAGCGACGAACAGCGATCGCTTCGATC

fdh-F

AATTCATTAAAGAGGAGAAAGGTACCATGAAGATCGTTTTAGTCTT

fdh-R

CATAGTTAATTTCTCCTACTAGTTCAGCCACCAATGATCGTGCGGATATC

phedh-F

CGATAAGAAATAAACTAGTAGGAGAAATTAACTATGAGCCTGGTGGAAAAAACCAGCA

phedh-R

GTTTTATTTGATGCCTCTAGAACTAGCTTAATTACGAATATCCCATTTCGGTTTAAC

xylBP.p-F

AATTCATTAAAGAGGAGAAAGGTACCACCAATCCGGAGTACCGGCTTAAG

xylBP.p-R

ATGCTGGTTTTAGTTAATTTCTCCTGCTAGCTTAATGGAAATCAAAGCAGCAAT

VecpZE-F

GAATTCATTAAAGAGGAGAAAGGTACCCCG

VecpZE-R

TGAGCCTTTCGTTTTATTTGATGCCTCTAGACTAG

VecpZA-F

AATTCATTAAAGAGGAGAAAGGTACCAAGCTTATGTTAAAGCGT

VecpZA-R

AGCCTTTCGTTTTATTTGATGCCGCTAGCTTAGTC