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Table 1 Strains and plasmids used in this study

From: Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. coliĀ from inexpensive aromatic precursors

Strains/plasmids

Phenotype

Source

Strains

Ā Ā 

BW25113

Ī”(araD-araB)567Ī”lacZ4787(::rrnB-3) Ī”lacZ4787(::rrnB-3) Ī”(rhaD-rhaB)568 hsdR514

CGSC

E. coli DH5Ī±

Host for plasmid construction

This study

ā€‰M1

BW25113 [pPLA-1]

This study

ā€‰M2

BW25113 [pPLA-2]

This study

ā€‰M3

BW25113 [pPLA-3]

This study

ā€‰M4

BW25113 [pPLA-4]

This study

ā€‰M5

BW25113 [pPLA-5]

This study

ā€‰M6

BW25113 [pPLA-6]

This study

ā€‰M7

BW25113 [pPLA-7]

This study

ā€‰M8

BW25113 [pPLA-6, pPLA-7]

This study

ā€‰M9

BW25113 [pPLA-6, pPLA-8]

This study

Plasmids

Ā 

This study

ā€‰pPLA-1a

pZE-PLlacO1-ltaEP.p-A8HB.t

This study

ā€‰pPLA-2a

pZE-PLlacO1-ltaEE.c-A8HB.t

This study

ā€‰pPLA-3a

pZE-PLlacO1-ltaEC.c-A8HB.t

This study

ā€‰pPLA-4a

pZE-PLlacO1-ltaEP.p-ilvAB.a

This study

ā€‰pPLA-5a

pZE-PLlacO1-ltaEP.p-TAAB.t

This study

ā€‰pPLA-6a

pZE-PLlacO1-ltaEP.p-A8HB.t-ridA

This study

ā€‰pPLA-7a

pZA-PLlacO1-fdh-pdh

This study

ā€‰pPLA-8a

pZA-PLlacO1-xylBP.p-pdh

This study

  1. aThe isopropyl-Ī²-D-thio-galactoside (IPTG) was required to induce the overexpression of introduced genes in plasmids