Fig. 7
Biosynthesis of L-phenylalanine from aromatic precursor benzyl alcohol. a Scheme showing the one-pot L-phenylalanine production from benzyl alcohol with an engineering cofactor NADH self-regeneration system. Production enzymes indicated the LtaEP.p, A8HB.t, and RidA that converted benzaldehyde to phenylpyruvate. b Biotransformation of the L-phenylalanine production in strain M9. Strain M9: BW25113 transformed with plasmids pPLA-6 and pPLA-8 to overexpress genes ltaEP.p, A8HB.t, ridA, pdh, and xylBP.p. Error bars are the standard deviation for three independent experiments