Fig. 3From: Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. coliĀ from inexpensive aromatic precursorsEffect of reaction conditions on the phenylpyruvate production by the enzyme cascade of LtaEP.p and A8HB.t combination. a The effects of reaction temperatures on the phenylpyruvate production. b The effects of reaction pH on the phenylpyruvate production. The following buffer systems were used: 100 mM Tris-HCl for pH 6.5, 7.5, 8.0, and 8.5. Error bars are the standard deviation for three independent experimentsBack to article page