Fig. 2
From: CO2-based production of phytase from highly stable expression plasmids in Cupriavidus necator H16
E. coli appA is expressed from different promoters and plasmids in C. necator H16 PHB-4. (A) AppA possesses classical phytase activity leading to the removal of one phosphate from plant derived phytic acid, and a phosphatase activity responsible for the release of up to four additional phosphate molecules. (B) Overview of the six native C. necator promoters tested for phytase production. Among them are the promoters of the two megaplasmid- and chromosomal-derived CBB-operons (cbb_pHG1 and cbb_chr), the promoters for the membrane bound and the soluble hydrogenase (mbh and sh), the promoter of a putative quorum sensing operon (phc) and the promoter of a fructose utilization operon (frc) (clockwise starting from the left, bottom position)