Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

Table 1 Sugar transporters identified in this work by LC/MS analysis

Protein ID	TMDs	pTM	Gravy values	Peptides	PSMs	Substrates	Refs.
STR1 (ID 50894)	12	0.85	0.42	5	30	Glc, Fru, Gal, Man, Xyl, LAra	[17, 20, 21]
65153	12	0.88	0.38	9	48
50618	12	0.75	0.15	18	213	Fru	[17]
CRT1 (ID 3405)	12	0.84	0.35	4	35	Glc, Sop, Cb, Lac	[18]
68812	12	0.84	0.23	12	222
HXT1 (ID 22912)	12	0.82	0.21	5	20	Glc	[19]
STP1 (ID 47710)	12	0.83	0.37	11	149	Cb, Glc	[10]
44175	12	0.83	0.23	10	119	Cb, Sop, Clt, Cltr	This work
80875	12	0.81	0.45	7	39
53903	12	0.89	0.39	2	5
121850	12	0.82	0.25	2	4
123702	12	0.75	0.42	6	64
76800	12	0.89	0.31	9	111
123809	12	0.79	0.70	2	10

The Table provides information about the sugar transporters identified in this work by LC/MS analysis, including: the Gravy Value (GV), the number of transmembrane domains (TMDs), predicted template modeling scores (pTM) for the predicted structures, distinct peptide sequences identified in the protein group, and the number of peptide spectrum matches (PSMs). The protein IDs correspond to the sugar transporters identification according with JGI database. The FDR was less than 1%. Known substrates of the sugar transporters are indicated. LAra: l-arabinose; Clt: cellotriose; Cltr: cellotetraose; Gal: d-galactose; Fru: d-fructose; Xyl: d-xylose; Glc: d-glucose; Lac: lactose; Cb: cellobiose; Man: d-mannose; Sop: sophorose

ISSN: 1475-2859