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Table 2 Primer pairs used during cloning of B  < oFabx(P40A) >and B <oFTN2(A40P)> 

From: RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling

Primer

Sequence

Fabx(P40A)_sense

5ʹ—GCGCCGGGCAAGGGC—3ʹ

Fabx(P40A)_antisense

5ʹ—CTGGCGGATCCAATCGACG-3ʹ

FTN2(P40A)_sense

5ʹ—CCGCCGGGTAAGGGTTT-3’

FTN2(P40A)_antisense

5ʹ—CTGGCGCACCCAGTT-3’

TN7_HO1

5ʹ—AGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAGTAGTAGGTTGAGGCCGTTG—3'

TN7_HO2

5ʹ—CAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCGGATATAGTTCCTCCTTTCAG—3ʹ

  1. The regions of the sequences introducing point mutations are underlined