Fig. 1

Final cell dry mass A, and intra- and extracellular soluble Fab yields B produced in different recombinant E. coli expression systems in fed-batch processes after 16 h of induction. Samples were analyzed in biological triplicates (n = 3, error bars represent standard error of the mean (SEM)). CDM of Fab-producing strains was compared to the respective wild-type strains BL21(DE3) and HMS174(DE3) (* … p < 0.05, ** … p < 0.01, *** p < 0.001, two sample t-test). The soluble Fab yields in the intra- and extracellular fractions were determined using ELISA. Fab expression patterns were analyzed in LC-specific WBs C and the ratio of LC to Fab in the soluble and IB fractions was estimated D