Table 6 The maximum rates of succinate growth-coupled production in iML1515 in rich medium (Strain0 in LB medium)
Number of knocked out reactions | Deleted reactions | Biomass formation rate (h−1) | Succinate production rate (mmol.gDW−1 h−1) | Deleted genes | Co-Knockout reactions |
|---|---|---|---|---|---|
Single | ARGDC | 1.08 | 19.72 | b4117 | – |
Double | ARGDC, FADRx | 1.05 | 22.09 | b4117, b3844 | FADRx, FE3Ri, FLVRx |
Triple | NDPK5, ASPTA, ARGDC | 1.03 | 28.14 | b0474, b2518, b0928, b4054, b4117 | ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, TYRTA, PHETA1, LEUTAi |
Quadruple | NDPK5, PFL, LDH_D, ACALD | 0.75 | 40.97 | b0474, b2518, b2579, b3952, b0902, b3951, b0903, b3114, b1380, b0351, b1241 | ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, OBTFL, ALCD2x, ALCD19 |