Table 5 The maximum rates of succinate growth-coupled production in iJR904 in rich medium (Strain0 in LB cultivation medium)
Number of knocked out reactions | Deleted reactions | Biomass formation rate (h−1) | Succinate production rate (mmol.gDW−1 h−1) | Deleted genes | Co-Knockout reactions |
|---|---|---|---|---|---|
Single | ADHEr | 1.35 | 20.10 | b1241 | LCADi_copy2 |
Double | F6PA, PFK | 1.28 | 33.69 | b0825, b3946, b3916, b1723 | − |
Triple | ACKr, GLCpts, PYK | 0.56 | 54.88 | b2296, b3115, b1849, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 | DHAPT, GART, PPAKr |
Quadruple | ACKr, ARGDC, GLCpts, PYK | 0.56 | 64.72 | b2296, b3115, b1849, b2938, b4117, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 | GART, PPAKr, DHAPTs |