Table 4 The maximum rates of succinate growth-coupled production in iML1515 (Strain0 in CM2 cultivation medium)
Number of knocked out reactions | Deleted reactions | Biomass formation rate (h−1) | Succinate production rate (mmol.gDW−1 h−1) | Deleted genes | Co-Knockout reactions |
|---|---|---|---|---|---|
Single | ATPS4rpp | 0.25 | 12.73 | b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739 | – |
Double | ATPS4rpp, PGL | 0.24 | 16.54 | b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739, b0767 | – |
Triple | PGI, ATPS4rpp, G6PDH2r | 0.17 | 23.16 | b4025, b3734, b3733, b3736, b3732, b3737, b3731, b3738, b3739, b3735, b1852 | – |
Quadruple | PFL, ACALD, THD2pp, THD2pp | 0.19 | 23.49 | b0351, b1241, b0903, b3951, b2579, b3952, b3114, b0902, b1602, b2913 | OBTFL, 'ALCD2x', 'ALCD19' |