Table 3 The guaranteed rate of succinate growth-coupled production in in iJR904 (Strain0 in CM2 cultivation medium)
Number of knocked out reactions | Deleted reactions | Biomass formation rate (h−1) | Succinate production rate (mmol.gDW−1 h−1) | SoGC (h−1) | Deleted genes | Co-knockout reactions | |
|---|---|---|---|---|---|---|---|
min | max | ||||||
Single | ADHEr | 0.16 | 5.11 | 9.50 | 1.41E-2 | b1241 | LCADi_copy2 |
Double | ADHEr, LDH_D | 0.15 | 8.08 | 9.51 | 1.43E-2 | b1241, b2133, b1380 | LCADi_copy2 |
Triple | ADHEr, LDH_D, PFL | 0.12 | 11.08 | 12.73 | 1.53E-2 | b1241, b2133, b1380, b3114, b0902, b3951 | LCADi_copy, OBTFL |
Quadruple | ADHEr, LDH_D, PFL, THD2 | 0.11 | 12.29 | 13.01 | 2.58E-2 | b1241, b2133, b1380, b3114, b0902, b3951, b1602 | LCADi_copy, OBTFL |
Quintuple | ADHEr, LDH_D, GLUDy, PFL, THD2 | 0.10 | 12.34 | 13.06 | 2.61E-2 | b1241, b2133, b1380, b1761, b3114, b0902, b3951, b1602 | LCADi_copy, OBTFL |