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Table 3 Seventeen trials of Box-Behnken design representing melanin production by S. djakartensis NSS-3

From: Bioproduction and optimization of newly characterized melanin pigment from Streptomyces djakartensis NSS-3 with its anticancer, antimicrobial, and radioprotective properties

Run No

Experimental parameters

Melanin yield (mg/10 mL)

A

B

C

Actual value

Predicted value

1

− 1

1

0

42.38

40.73

2

0

− 1

− 1

60.75

77.59

3

1

0

− 1

89.51

72.67

4

0

1

− 1

99.20

100.85

5

0

1

1

60.50

65.80

6

0

0

0

105.50

92.32

7

0

− 1

1

50.22

63.40

8

1

− 1

0

107.22

101.92

9

0

0

0

75.30

71.65

10

0

0

0

110.12

121.66

11

0

0

0

109.20

97.66

12

1

0

1

99.20

102.85

13

0

0

0

105.20

103.44

14

− 1

− 1

0

102.40

103.44

15

1

1

0

98.75

103.44

16

− 1

0

− 1

106.33

103.44

17

− 1

0

1

104.50

103.44