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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Heterologous production of 3-hydroxypropionic acid in Methylorubrum extorquens by introducing the mcr gene via a multi-round chromosomal integration system based on cre-lox71/lox66 and transposon

Fig. 5

Comparative analysis of fluorescence and 3-HP detection of all the constructed strain isolates. (a) Heatmap of RFI generated from 50 picked isolates of all constructed strains. The numbers on the left of each heatmap column represent the strain isolates, and the color column on the upper right indicates the RFI values (GFP/OD600). (b) 3-HP production of the top 10 isolates of each strain based on the RFI, and strain AM1-MG80 (4 isolates) was used as control. (c) qRT-PCR analysis of transcriptional expression of the mcr gene in all the constructed strains. The expression level of genes in AM1-MG80 strains was set as 1. the t-test was used to calculate the statistical significance by pairwise comparison. * 0.01 < P < 0.05; **P < 0.01

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Microbial Cell Factories

ISSN: 1475-2859

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