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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Heterologous production of 3-hydroxypropionic acid in Methylorubrum extorquens by introducing the mcr gene via a multi-round chromosomal integration system based on cre-lox71/lox66 and transposon

Fig. 1

Schematic diagram of vectors constructed in this study. (a) pSL-TP-cre-km; (b) pCM80-mcr-egfp; (c) pTP-cre-mcr-egfp; (d) pTP-mcr-egfp

Tpase: miniHimar transposase. IR: insertion sequence recognized by miniHimar transposase. TrrnB: transcriptional terminator. PR/tetO: inducible promoter by aTc. cre: gene coding for Cre recombinase. kmr: kanamycin resistance gene. egfp: gene coding for green fluorescent protein. tet: tetracycline resistance gene. M13 ori: high-copy replicon origin for E. coli. p15A ori: low-copy replicon origin. lox71and lox66: the lox sites for recombination by Cre

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Microbial Cell Factories

ISSN: 1475-2859

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