Fig. 2
From: Enhanced protein secretion in reduced genome strains of Streptomyces lividans
Total biomass and native secretome yield in S. lividans TK24 and reduced genome derivative strains. (A) Dry cell weight (DCW; g/L; black bars) and total secretome yield (mg secreted protein/ g DCW; grey bars) following growth of the indicated strains in nutrient broth (NB) until late exponential phase. n = 3; values represent the mean ± SD; (B) Equal amount of secreted polypeptides (3 µg/lane) from the indicated strains, grown as in A, were analyzed by SDS-PAGE and silver stained. Lane 1: molecular weight marker. Proteins with major (arrows)/ little (asterisks) change among strains are indicated