Table 2 PLSR analysis of offline (at-line) HTS-FT-Raman spectral data
Analysis | Control | Bypass loop | ||||
|---|---|---|---|---|---|---|
Range | R2CV (A) | RMSE | Range | R2CV (A) | RMSE | |
Rhodotorula | ||||||
Glucose (g/L) | 0.04–53.81 | 0.93 (2) | 4.86 (9.0%) | 0.03–52.54 | 0.91 (2) | 5.65 (10.7%) |
Cell dry weight (g/L) | 0.32–18.60 | 0.94 (2) | 1.47 (7.9%) | 0.32–18.01 | 0.93 (2) | 1.47 (8.2%) |
Total lipids (%w/w) | 4.96–65.54 | 0.98 (3) | 3.08 (4.7%) | 5.57–67.04 | 0.98 (3) | 3.24 (4.8%) |
Total lipids (g/L) | 0.02–12.18 | 0.90 (2) | 1.33 (10.9%) | 0.02–11.98 | 0.88 (2) | 1.42 (11.9%) |
Total carotenoids (‰w/w) | 0–11.88 | 0.88 (2) | 1.51 (12.7%) | 0–12.62 | 0.88 (1) | 1.66 (13.1%) |
Total carotenoids (mg/L) | 0–162.23 | 0.97 (2) | 11.06 (6.8%) | 0–166.51 | 0.99 (3) | 6.3 (3.8%) |
Schizochytrium | ||||||
Glycerol (g/L) | 12.53–53.75 | 0.93 (4) | 3.29 (6.1%) | 13.86–54.17 | 0.94 (4) | 2.78 (5.1%) |
Cell dry weight (g/L) | 2.36–26.56 | 0.76 (2) | 3.23 (12.2%) | 2.98–26.31 | 0.90 (4) | 2.05 (7.8%) |
Total lipids (%w/w) | 13.97–56.13 | 0.89 (4) | 4.48 (8.0%) | 10.87–41.50 | 0.60 (1) | 3.99 (9.6%) |
Total lipids (g/L) | 0.33–13.87 | 0.76 (2) | 1.81 (13.1%) | 0.32–9.22 | 0.87 (4) | 1.44 (15.6%) |
Total carotenoids (‰w/w) | 0.05–0.54 | 0.50 (1) | 0.11 (20.4%) | 0.03–0.39 | 0.19 (1) | 0.15 (38.5%) |
Total carotenoids (mg/L) | 0.16–8.63 | 0.81 (3) | 1.15 (17.5%) | 0.21–8.87 | 0.81 (5) | 0.97 (10.9%) |