Table 1 Oligonucleotide primers and plasmid templates used for amplification of DNA sequences coding for chimeric LPP′-OmpA′, SUMO, Δ59 Sortase A and LPETG-PEN-HuscFv34
Gene | Primer (5′ to 3′) | Plasmid template | |
|---|---|---|---|
Forward | Reverse | ||
LPP′-OmpA′ | GGAGATATACCATGGCGATGAAAGCTACTAAACTG | ATTGACTTCTGAGTCGGTACCAAGCTTGTCGACTC | Lpp′-OmpA′-LPQPG-aa3h-huscfv34-pET28b+ |
SUMO | GGAGTCGACAAGCTTGGTACCGACTCAGAAGTCAAT | CTGCGGTTTTGCCTGGGTACCGCCACCAATCTGTTC | Lpp′-OmpA′-SUMO-huscfv34-pET28b+ |
∆59 Sortase A | GAACAGATTGGTGGCGGTACCCAGGCAAAACCGCAG | GCCGGTCTCGGGCAGTTTCACTTCGGTTGC | Sortase A-Del59-pET28a+ |
LPETG-pen-huscfv34 | GCAACCGAAGTGAAACTGCCCGAGACCGGC | TGGTGGTGCTCGAGTTATTAGTGGTGGTG | Lpp′-OmpA′-pen-huscfv34-his-pET24a+ |