Fig. 6

Biocompatibility and cell-penetrating ability of the G-PEN-HuscFv34-6× His. (a) Percent viability of cells: Vero (blue bars)/Vero E6 (olive bars) after treatment with different concentrations of G-PEN-HuscFv34-6× His at 37 °C in 5% CO₂ incubator for 24 h. (b) Left block: Intracellular localization of the G-PEN-HuscFv34-6× His after adding 0.5 μM to the Vero E6 cells, incubated, fixed, permeated and probed with mAb to HuscFv34 followed by goat anti-mouse Ig-Alexa Fluor 488 and DAPI. The G-PEN-HuscFv34-6× His appears green; the nuclei stained blue; the cell contours are indicated by dotted lines. Right block, negative control (cells in medium instead of the G-PEN-HuscFv34-6x His and probed with mAb to HuscFv34)