Fig. 4

Suitable temperature for growing 0.025 mM IPTG-induced transformed bacteria to express the fusion protein. The transformed BL21 (DE3) ΔA competent E. coli was grown under 0.025 mM IPTG induced condition at 16 and 37 °C for 5 h. The cells were then stained as in Fig. 3 and analyzed for percent surface displayed LPP′-OmpA′-SUMO-Δ59 Sortase A-LPETG-PEN-HuscFv34-6× His by flow cytometry. The transformed bacteria cultured at 37 °C expressed higher percentage of the cells displaying the fusion protein on surface (61.1%) than when they were grown at 16 °C (28.1%)