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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system

Fig. 2

Indirect ELISA titers of mAbs in spent culture supernatants of hybridomas raised against HuscFv34. HuscFv34 (1 μg per well) was used as antigen in the indirect ELISA. Homogenate of original BL21 (DE3) E. coli was used as control antigen. Mouse immune serum (mouse anti-HuscFv34 pAb) at dilution 1:5000 served as positive antibody control. X axis, culture supernatant of hybridoma clones; Y axis indirect ELISA OD 405 nm against blanks (wells added with PBS instead of antibody). The dotted line is the arbitrarily cut-off level (OD 405 nm = 0.2) between positive and negative indirect ELISA results

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Microbial Cell Factories

ISSN: 1475-2859

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