Table 6 Strains and plasmids
Strains and plasmids | Description | References |
|---|---|---|
Strains | ||
 E. coli DH5α | Strain for plasmid amplification | Invitrogen |
 E. coli ET12567 | Strain harbouring pUZ8002 for conjugal gene transfer | [23] |
 E. coli BL21 (DE3) | Strain for high-level expression of recombinant proteins | Thermo Fisher Scientific |
 E. coli p21pcaI | Derivative of E. coli BL21 (DE3) harbouring p21pcaI gene | This work |
 E. coli p21pcaJ | Derivative of E. coli BL21 (DE3) harbouring p21pcaJ gene | This work |
 S. lividans ΔYA8 | Derivative of S. lividans TK24, 8 secondary metabolite gene clusters were removed from genomic DNA | [18] |
 S. lividans ΔYA8-DG2 | Derivative of S. lividans ΔYA8 containing bottromycin biosynthetic cluster | This work |
 S. lividans ΔYA8-R2 | Derivative of S. lividans ΔYA8 containing pamamycin biosynthetic cluster | This work |
Plasmids | ||
 pET21a | Vector for protein overexpression | Invitrogen |
 p21pcaI | Derivative of pET21a plasmid. Protein expression vector for pcaI gene of P. putida KT2440 | This work |
 p21pcaJ | Derivative of pET21a plasmid. Protein expression vector for pcaJ gene of P. putida KT2440 | This work |
 DG2-Km-P41hyg | Derivative of integrative DG2-cosmid with KmR marker and P41 promoter pairs, contains bottromycin biosynthetic cluster | [20] |
 R2 | An Integrative cosmid containing pamamycin biosynthetic gene cluster | [38] |