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Table 4 Expression levels of genes involved in gluconeogenesis, the phosphoenolpyruvate-pyruvate-oxaloacetate node and the ethylmalonyl-CoA pathway (log2 fold change). The strain was grown on minimal medium with 10 mM mannitol, protocatechuate (PCA), or 4-hydroxybenzoate (4HB) as the sole carbon source. Samples were taken from the cultures after 12 h (Fig. 3). The expression levels are normalized to the mannitol-based culture and are given as log2-fold change. The significance level (Benjamini‒Hochberg, FDR) was set to < 0.05, n = 3

From: From waste to health-supporting molecules: biosynthesis of natural products from lignin-, plastic- and seaweed-based monomers using metabolically engineered Streptomyces lividans

Gene

Annotation

PCA

4HB

Gluconeogenesis

 SLYA8N_13150

Fructose-1,6-bisphosphatase

2.06

2.98

 SLYA8N_13500

Phosphoenolpyruvate carboxykinase [GTP]

3.34

3.28

 SLYA8N_35210

Pyruvate carboxylase

1.12

0.46

Phosphoenolpyruvate-pyruvate-oxaloacetate node

 SLYA8N_11390

Pyruvate kinase

− 1.28

− 1.05

 SLYA8N_12060

Putative NAD-dependent malic enzyme

1.14

2.06

 SLYA8N_22045

Phosphoenolpyruvate carboxylase

− 0.90

− 3.63

 SLYA8N_22940

NAD-dependent malic enzyme

0.16*

0.82

 SLYA8N_25215

Pyruvate phosphate dikinase

5.70

6.14

 SLYA8N_27640

Pyruvate kinase

− 1.03

− 1.57

Ethylmalonyl-CoA pathway

 SLYA8N_04575

Fatty oxidation protein

0.21*

− 0.31*

 SLYA8N_04850

Fatty acid oxidative multifunctional enzyme

− 0.52

− 1.04

 SLYA8N_04855

Putative acyltransferase

− 0.33*

− 1.27

 SLYA8N_06050

Oxidoreductase

3.87

3.31

 SLYA8N_06055

Transcriptional regulator

0.41*

− 0.41*

 SLYA8N_06060

Crotonyl-CoA reductase

6.28

3.55

 SLYA8N_06065

Protein MeaA

4.29

3.42

 SLYA8N_06070

Citrate lyase

2.89

2.24

 SLYA8N_06075

Hypothetical protein

2.98

2.68

 SLYA8N_06080

Acyl-CoA dehydrogenase

1.58

1.15

 SLYA8N_06790

Acetyl-/propionyl-coenzyme A carboxylase alpha chain

0.74

− 0.61

 SLYA8N_11430

Isobutyryl-CoA mutase A

0.30*

1.42

 SLYA8N_11500

Putative acetyl-CoA acetyltransferase

0.65

1.47

 SLYA8N_11505

Hypothetical protein

1.03

0.57

 SLYA8N_11570

3-Hydroxybutyryl-CoA dehydrogenase

0.58

1.29

 SLYA8N_13745

Propionyl-CoA carboxylase beta chain

0.80

− 0.56

 SLYA8N_13770

Acetyl-/propionyl-coenzyme A carboxylase alpha chain

0.58

− 0.60

 SLYA8N_14030

Methylmalonyl-CoA mutase

0.51

1.30

 SLYA8N_14360

Isobutyryl-CoA mutase small subunit

0.42

0.34

 SLYA8N_23780

Putative acyl-CoA dehydrogenase

1.24

1.57

 SLYA8N_23785

Hydroxymethylglutaryl-CoA lyase

1.09

1.61

 SLYA8N_23790

Acetyl-/propionyl-coenzyme A carboxylase alpha chain

0.99

1.26

 SLYA8N_23795

Methylcrotonoyl-CoA carboxylase beta chain

1.35

1.51

  1. * Genes not significant (Benjamini‒Hochberg FDR > 0.05) but included for completeness
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Microbial Cell Factories

ISSN: 1475-2859

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