Table 2 Strains and plasmids used in this study
Strain or plamid | Characteristics | Reference or source |
|---|---|---|
Strains | Â | Â |
E. coli | Â | Â |
DH5α | General cloning host | Invitrogen |
ET12567/pUZ8002 | Donor strain for intergeneric conjugation | [39] |
S. lincolnensis | Â | Â |
SyBE2901 | Original strain for high lincomycin-producer, derived from ATCC25466 | ATCC25466 |
ST708 | SyE2901 ∆SLCG_7083 | This study |
ST717 | SyBE2901 with pLCY010-7083 | This study |
ST718 | ST708 with pLCY010-7083 | This study |
Plasmids | Â | Â |
pKCcas9dO | acc(3)IV, pSG5, tipA-Scocas9, j23119, actII-orf4 guide-RNA, homologous region flanking act-orf4 | [36] |
pLCY3208 | acc(3)IV, pSG5, tipA-Scocas9, j23119, SLCG_7083 guide-RNA, homologous region flanking SLCG_7083 | This study |
pLCY010 | pUWL201 derivative, ampr, tsrr, hygr, carrying PermE* promoter | [35] |
pLCY010-7083 | pLCY010 with PermE*SLCG_7083 | This study |