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Fig. 2 | Microbial Cell Factories

Fig. 2

From: PAS domain containing regulator SLCG_7083 involved in morphological development and glucose utilization in Streptomyces lincolnensis

Fig. 2

Construction of SLCG_7083 disruption mutant ST708. (a) Schematic representation of the CRISPR/Cas9-mediated cleavage of genomic DNA and homology directed repair (HDR) to delete of SLCG_7083. (b) confirmation of the SLCG_7083 deletion strain ST708 by PCR amplification. Lane 1: a 3187-bp amplicon using the original SyBE2901 genomic DNA as a template; Lane 2: a 2566-bp amplicon using the ST708 genomic DNA as a template; M: DNA ladder. (c) Sequencing confirmation of the amplicon from ST708 strain

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Microbial Cell Factories

ISSN: 1475-2859

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