Fig. 6

New versions of precursor plasmids created by swapping out the original integration array. PCR products (see agarose gel) from eDA197 or eDA227, digested with AfeI and XhoI were ligated into appropriately digested eDA155. Precursors v2A and v2B were linearized with I-SceI then used to transform wild-type or ΔKU70 cells to create a total of four strains (see also Additional file 1: Fig. S1)