Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform

Table 3 Extracellular cordycepin production by microorganisms in submerged fermentation

Organisms	Strain*	Cultivation mode	Precursor supplement (g/L)**	Cultivation time (d)	COR titer (mg/L)	COR productivity (mg/L/d)	References
A. oryzae	Recombinant	Batch	Adenine (1)	2	1,129.29	564.64	This study
Y. lipolytica	Recombinant	Fed-batch	Adenine (1)	7	4,365.54	623.23	[50]
	Recombinant	Batch	Adenosine	9	3,588.51	398.33	[21]
K. phaffii	Recombinant	Batch	Adenine (2)	7	2,680.00	382.86	[45]
S. cerevisiae	Recombinant	Batch	n/a	n/a	137.27	n/a	[22]
E. coli	Recombinant	Batch	n/a	n/a	nd	n/a	[20]
C. militaris	Wild-type	Batch	n/a	18	345.40	19.20	[53]
	Wild-type	Repeated batch	Adenine (1) +Glycine (16)	75	2,350.00	188.00	[48]
	Mutant	Repeated batch	Adenosine (6)	30	8,600.00	290.00	[18]
	Mutant	Batch	n/a	45	14,300.00	317.00	[54]
	Recombinant	Batch	Glycine (1)	12	2,581.96	215.16	[13]
Irpex lacteus	Wild-type	Batch	n/a	13	162.05	12.47	[55]

*Recombinant strains were constructed by overexpressing a set of genes involved in cordycepin (COR) biosynthesis. The mutant strain was constructed using proton beam irradiation
**n/a, not available; nd, not detected

ISSN: 1475-2859