Fig. 6From: Selective extraction of recombinant membrane proteins from Hansenula polymorpha by pulsed electric field and lytic enzyme pretreatmentSDS PAGE (A) and Western blot (B) analysis of the proteins extracted from PEF treated cells before and after XAD 4 and KSCN treatment. Lane M, molecular weght marker; lane 1, extracts from cells incubated with 0.5% Triton X-100 for 75Â min at 39 oC; lane 2, extracts from cells incubated with 0.5% Triton X-100 at room temperatureBack to article page