Fig. 5

Effect of the preincubation of the cell suspension at 60 ^oC on the extraction efficiency. (A) Lane M, molecular weight marker; lane 1, extracts obtained after incubation with 0.5% Triton X-100 for 75 min at 42 ^oC; lane 2, extracts obtained from cells preincubated for 10 min at 60 ^oC before Triton X-100 addition (0.5% Triton Х-100, 75 min at 42 ^oC); (B) extracts obtained from cells preincubated for 10 min at 60 ^oC and after that incubated with Triton X-100 at room temperature or at 42 ^oC. The host proteins are indicated with asterisk