Fig. 4

SDS PAGE analysis of the proteins released after incubation with lyticase and after the following treatment with detergent. Lane M, molecular weght marker; lane 1, proteins released from PEF treated cells after 2 h incubation in 125 mM PPB pH 8 with 20 U mL^− 1 lyticase and 5 mM DTT; 2, proteins released from control cells under the same conditions of incubation; 3, proteins released after incubation of PEF treated cell with 0.5% Triton X-100 (75 min, 39 ^oC); 4, the same extract as in 3 after 10 min incubation at 60 ^oC; 5, proteins released from control cells incubated with 0.5% Triton X-100 (75 min, 39 ^oC); 6, the same extract as in 5 after 10 min incubation at 60 ^oC