Fig. 3

SDS PAGE analysis of: (A) extracts obtained after incubation of PEF treated cells with 0.5% Triton X-100 for 75 min at 42 ^oC and 45 ^oC; (B) soluble fractions (lanes 1 and 2) and insoluble fraction (lane 3) obtained after treatment of electropermeabilized cells preincubated with 30 U mL^− 1 lyticase for 3 h at 42 °C with 0.5% Triton X-100. Lane 2 presents the same extract (lane 1) after incubation for 10 min at 60 ^oC and centrifugation to remove the denaturated proteins; (C) soluble fractions obtained after treatment of cell suspensions (125 mM PPB pH 8) with 0.5% Triton X-100 for 75 min at 42 ^oC: lane1, electropermeabilized cells preincubated for 2 h with 5 mM DTT; lane 2, control cells preincubated for 2 h with 5 mM DTT; lane 3, electropermeabilized cells preincubated for 2 h with 20 U mL^− 1 lyticase/5 mM DTT