Fig. 2

SDS PAGE (А) and Western blot (B) analysis of extracts from electrically treated cells obtained after 75 min incubation with 0.5% Tween 20 or 0.5% Triton X-100. Lane M, molecular weight marker; lane 1, soluble fraction obtained after incubation of the cell suspension (125 mM PPB, pH 8) for 75 min with 0.5% Tween 20 at room temperature; lane 2, 0.5% Тriton X-100 at room temperature; lane 3, 0.5% Tween 20 at 39 ^oC; lane 4, 0.5% Тriton X-100 at 39 ^oC. The same volumes of extracts obtained from cell suspensions with equal cell concentrations are loaded on to each lane