Fig. 10From: Selective extraction of recombinant membrane proteins from Hansenula polymorpha by pulsed electric field and lytic enzyme pretreatment(A) SDS PAGE of the proteins released from PEF treated cells after 90Â min incubation with Triton X-100 at different temperatures; (B) proteins released from PEF treated cells after incubation with 0.5% Triton X-100 at RT; (C) cComparrison of the yields of dS obtained from PEF treated (1) and control (2) cells under different conditions of incubation with 0.5% Triton X-100; (D) Western blot analysis of the soluble fractions obtained after mechanical disruption of the cells and ON incubation with Tween 20 at 4 oC, and the proteins released from PEF treated cells after incubation with 0.5% Triton X-100, ON at 4 oCBack to article page