Fig. 1From: Selective extraction of recombinant membrane proteins from Hansenula polymorpha by pulsed electric field and lytic enzyme pretreatmentProtein release from control and electrically treated cells. (A) after incubation for 2 h at 38 °C with 20 U mL− 1 lyticase/5 mM DTT or only with 5 mM DTT; (B) after subsequent incubation for 16 h at 4 °C in 125 mM PPB pH 8. The values represent the mean ± SD of five different experimentsBack to article page