Table 1 Selected gene candidates predicted by different design algorithms in various media conditions
Target Gene | Design algorithm | Target compound | Carbon source | Intervention |
|---|---|---|---|---|
DPP1 (YDR284C) | OptGene | Acetyl-CoA | Glucose | Knock-out |
OAR1 (YKL055C) | OptGene | GGPP | Galactose | Knock-out |
MDH3 (YDL078C) | OptKnock & OptForce | Acetyl-CoA | Glucose | Knock-out |
ACH1 (YBL015W) | OptKnock & OptGene | Acetyl-CoA | Glucose | Knock-out |
MLS1 (YNL117W) | OptKnock | Acetyl-CoA | Galactose | Knock-out |
DIT1 (YDR403W) | OptGene | Acetyl-CoA | Glucose | Knock-out |
LPP1 (YDR503C) | OptGene | GGPP | Glucose & galactose | Knock-out |
ISC1 (YER019W) | OptGene | Acetyl-CoA | Galactose | Knock-out |
DTR1 (YBR180W) | OptGene | GGPP | Glucose | Knock-out |
ILV2 (YMR108W) | OptForce | Acetyl-CoA | Galactose | Overexpression |
TRR1 (YDR353W) | OptForce | Acetyl-CoA | Galactose | Overexpression |
ADE4 (YMR300C) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |
ADE5,7 (YGL234W) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |
ADE13 (YLR359W) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |
ECM31 (YBR176W) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |
CAB1 (YDR531W) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |
SPE2 (YOL052C) | OptForce | Acetyl-CoA | Glucose & galactose | Overexpression |