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Table 1 Parameters of whole cell catalyst and reaction for synthesis of 3SL

From: Modular bioengineering of whole-cell catalysis for sialo-oligosaccharide production: coordinated co-expression of CMP-sialic acid synthetase and sialyltransferase

 

Activity (protein) ratio CSS:α2,3-SiaT

catalyst concentration [gcdm/L]a

3SL [g/L]

Specific production rate [g 3SL/gcdm/h]c

STY [g/(L h)]d

TTN [–]e

pBICI_weak (113 mg/gcdm)

1:1 (1:6.3)

0.58

8.2 (69)b

16

6.2

14

pBICI_strong (145 mg/gcdm)

1:0.3 (1:1.9)

0.22

5.1 (42)

23

4.0

23

pDUAL_tacI (175 mg/gcdm)

1:3 (1:19)

0.94

8.5 (68)

13

8.1

9.0

pDUAL_T5 (119 mg/gcdm)

1:2 (1:13)

0.98

8.3 (70)

11

7.5

8.5

  1. aCell concentration used for CSS activity of 0.4 U/mL; 37 °C, 20 mM substrate
  2. b3SL yield (based on 20 mM substrate used) shown in brackets
  3. cCalculated for the 30 min reaction
  4. dCalculated for the 1 h reaction
  5. eTTN = g3SL after 2 h/gcdm
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Microbial Cell Factories

ISSN: 1475-2859

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