Fig. 6
Enzyme stability in whole cell catalysts. A α2,3-SiaT and B CSS in pBICI_strong. The catalyst was incubated (pH 8.0, 450 rpm) at 37 °C (squares) or 25 °C (triangles). Activities (photometric assay) are mean values of three replicate experiments and error bars show the S.D. Symbols show the data and dashed lines indicate the trend. The inset of panel A shows exponential fit (dashed blue line) of the data. C Simulation of 3SL release in the absence of (dotted lines) and under the assumed involvement of inactivation of α2,3-SiaT (dashed lines), compared to experimental data at 37 °C. A first-order inactivation constant of 6.1 × 10−3 min−1 (equivalent to a half-life of 110 min) was assumed