Fig. 4
Reaction time courses of whole cell-catalyzed 3SL synthesis. The reactions are performed with ~ 20 mM of each Neu5Ac, CTP and lactose at 37 °C and pH 8.0. The CSS activity is normalized to 0.40 U/mL in each reaction based on variable loading of the cell catalyst, hence also activity ratio (RE) of CSS:α2,3-SiaT, as follows. A, B 0.22 gcdm/L, pBICI_strong, RE 1:0.3; C, D 0.58 gcdm/L, pBICI_weak, RE 1:1; E, F 0.94 gcdm/L, pDUAL_tacI, RE 1:3; G, H 0.98 gcdm/L, pDUAL_T5, RE 1:2). Symbols show the mean values of 3 replicate experiments and error bars show the S.D