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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Modular bioengineering of whole-cell catalysis for sialo-oligosaccharide production: coordinated co-expression of CMP-sialic acid synthetase and sialyltransferase

Fig. 3

Co-expression results for CSS and α2,3-SiaT analyzed by enzyme activity (A) and SDS PAGE (B). The expression was here done at 25 °C. HPLC assay was used for activity determination (see “Methods”) at 37 °C. The SDS polyacrylamide gel shows the soluble fraction received by E. coli cell disruption. CSS (26.1 kDa) and α2,3-SiaT (47.2 kDa) are highlighted with arrows. Assignment of protein band to enzyme was based on molecular mass. Activity measurements shown in panel A confirm the presence of the enzyme. Molecular mass standard (Std); pBICI_weak (W); pBICI_strong (S); pDUAL_tacI (tacI); pDUAL_T5 (T5), empty vector (EV)

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Microbial Cell Factories

ISSN: 1475-2859

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