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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Modular bioengineering of whole-cell catalysis for sialo-oligosaccharide production: coordinated co-expression of CMP-sialic acid synthetase and sialyltransferase

Fig. 2

Kinetic simulations of whole cell-catalyzed production of 3SL. The 3SL release in reactions of 20 mM substrate (CTP, Neu5Ac, lactose) is shown for an assumed E. coli cell that co-expresses 150 mg total recombinant protein/gcdm in variable relative composition of α2,3-SiaT and CSS. The specific activity of the enzymes (CSS: 36 U/mg; α2,3-SiaT: 5.7 U/mg) leads to the activity ratios shown in A. Reactions for 2 h at variable cell loadings. The dashed vertical line shows results for a perfectly balanced enzyme ratio. B Time courses of 3SL release at varied activity ratio. C Analysis of minimum time required for maximum 3SL release (16 mM). D Effect of enzyme ratio on time to maximum 3SL and on 3SL remaining after 2 h, determined for a catalyst loading of 0.80 gcdm/L

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Microbial Cell Factories

ISSN: 1475-2859

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