Table 1 Activity screening of whole-cell lysates of E. coli NEB5Ī± expressing the individual enzymes for the two-step conversion of ferulic acid into coniferyl alcohol via coniferyl aldehyde
From: Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols
Enzyme | Substrate | Product yield | |
|---|---|---|---|
Coniferyl aldehyde (%) | Coniferyl alcohol (%) | ||
NiCAR | Ferulic acid | 4.6 | 0.02 |
SrCAR | Ferulic acid | 28.2 | 0.26 |
PsCAD | Coniferyl aldehyde | - | 14.5 |
MsCAD2 | Coniferyl aldehyde | - | 19.3 |
CgAKR1 | Coniferyl aldehyde | - | 4.5 |
NiCARā+āPsCAD | Ferulic acid | 3.3 | 17.9 |
NiCARā+āMsCAD2 | Ferulic acid | 1.1 | 33.6 |
NiCARā+āCgAKR1 | Ferulic acid | 2.3 | 13.3 |
SrCARā+āPsCAD | Ferulic acid | 12.1 | 26.0 |
SrCARā+āMsCAD2 | Ferulic acid | 6.3 | 50.3 |
SrCARā+āCgAKR1 | Ferulic acid | 20.2 | 8.4 |