Fig. 3
From: Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols
Bioconversion of ferulic acid into eugenol using the CAR-dependent pathway. A The eugenol biosynthesis pathway is split into two modules. The first module consists of SrCAR and MsCAD2 enzymes, catalysing the two-step reduction of ferulic acid, resulting in the formation of coniferyl alcohol. The second module comprises PhCFAT and ObEGS enzymes, catalysing the two-step conversion of coniferyl alcohol into eugenol. B Production of eugenol in E. coli NEB5Ī± carrying SBC009876 in combination with one of the three indicated plasmids. At time point zero, cells were supplemented with 3 mM ferulic acid, and expression of pathway enzymes was induced by addition of IPTG. Error bars represent standard deviations of biological triplicates