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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila

Fig. 5

SDS-PAGE analysis of the extracellular proteins of the M. thermophila strains. (A) Overexpression of MtCbh-1, MtGh5-1, MtLPMO9B, and MtEpl1. Lane M, molecular mass standard; Lane 1, the parent ∆Mtalp1 strain; Lane 2, Mtepl1-expressing strain; Lane 3, MtLPMO9B-expressing strain; Lane 4, Mtgh5-1-expressing strain; Lane 5, Mtcbh-1-expressing strain. Red arrows indicate the protein band corresponding to MtEpl1, MtLPMO9B, MtGh5-1, and MtCbh-1, respectively. (B) Overexpression of TrGlaA. Lane 1, the parent ∆Mtalp1 strain; Lane 2, TrglaA-expressing strain. The red arrow indicates the protein band corresponding to TrGlaA. All strains were cultured in fermentation medium for 3 days

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Microbial Cell Factories

ISSN: 1475-2859

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