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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila

Fig. 2

Expression of homologous laccase in M. thermophila under the control of different promoters. (A) Schematic representation of the gene expression cassettes for lcc1. (B) Laccase activity in culture supernatants of M. thermophila strains. (C) SDS-PAGE analysis of proteins secreted by transformants carrying (lane 1 and 4) or not carrying (lane 2 and 3) the Ppdc-lcc1 expression cassette. 50 µL of culture supernatant sample was loaded in each lane for Coomassie staining. The glycosylated MtL encoded by lcc1 is indicated by an arrow. All strains were cultured in fermentation medium at 45 °C and 200 rpm for 3 days

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Microbial Cell Factories

ISSN: 1475-2859

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