Fig. 1

Efficient laccase production under glucose-repressed conditions. (A) lcc1-expressing strains were identified by the ABTS plate assay. Transformants were inoculated onto the MM medium supplemented with 1 mM ABTS and incubated at 37 °C for 3 h. (B) Laccase activity in culture supernatants of ∆Mtalp1::Ptef1-lcc1-Tlcc1 strain after cultivation in fermentation medium containing 4% glucose or cellulase-inducing medium containing 2% Avicel (MM + 2% Avicel) at 45 °C and 200 rpm for 3 days