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Table 2 Comparison of theoretical performance of iMT1026 v3.0 with experimental data from the methanol fed-batch cultivations at µ = 0.03 h−1

From: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid

 

PpCβ20

PpCβ21

PpCβ21-P

Predicted YP/S

0.16

0.17

0.16

Experimental YP/S

0.14 ± 0.01

0.15 ± 0.01

0.15 ± 0.01

Relative deviation (%)

6–19

6–18

0-12.5

Predicted YP/X

0.57

0.68

0.77

Experimental YP/X

0.52 ± 0.03

0.61 ± 0.03

0.69 ± 0.03

Relative deviation (%)

3.5–14

6–15

6.5–14

  1. 3-HP yield on methanol (YP/S) (g3-HP gMetOH−1), 3-HP yield on biomass (YP/X) (g3-HP gDCW−1). ± indicates SD of the biological replicates
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Microbial Cell Factories

ISSN: 1475-2859

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