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Table 1 Averaged value of key process parameters obtained for the methanol fed-batch phase using a pre-programmed µ of 0.03 h−1

From: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid

 

PpCβ20

PpCβ21

PpCβ21-P

QP (g3-HP l−1 h−1)

0.46 ± 0.01

0.48 ± 0.02

0.46 ± 0.01

YX/S (gDCW gMetOH−1)

0.28 ± 0.01

0.25 ± 0.01

0.21 ± 0.01

YP/S (g3-HP gMetOH−1)

0.14 ± 0.01

0.15 ± 0.01

0.15 ± 0.01

YP/X (g3-HP gDCW−1)

0.52 ± 0.03

0.61 ± 0.03

0.69 ± 0.03

YCO2/S (gCO2 gMetOH−1)

0.70 ± 0.02

0.73 ± 0.02

0.82 ± 0.03

qS (gMetOH gDCW−1 h−1)

0.103 ± 0.002

0.107 ± 0.002

0.124 ± 0.002

qP (mmol3-HP gDCW−1 h−1)

0.166 ± 0.001

0.183 ± 0.001

0.201 ± 0.001

qCO2 (mmolCO2 gDCW−1 h−1)

1.65 ± 0.01

1.79 ± 0.01

2.30 ± 0.01

µ (h−1)

0.029 ± 0.001

0.027 ± 0.001

0.026 ± 0.001

  1. Volumetric productivity (QP), biomass yield on methanol (YX/S), 3-HP yield on methanol (YP/S), 3-HP yield on biomass (YP/X), CO2 yield on methanol (YCO2/S), specific substrate consumption rate (qS), specific 3-HP production rate (qP), specific carbon dioxide evolution rate (qCO2), and experimentally measured mean specific growth rate (µ). Cultivations were performed in duplicate and biomass concentration analyses were performed in triplicate. ± indicates SD of the biological replicates