Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Table 4 Statistical analysis for FCCD results of cyanobacterial alginate production from S. algini MNE ON864447

Source	Sum of squares	df	Mean Square	F-value	p-value	Coefficient estimate
Model	8.47	9	0.9410	303.79	< 0.0001	1.45
A-Working volume	0.3322	1	0.3322	107.25	< 0.0001	−0.1560
B-Incubation period	0.4884	1	0.4884	157.68	< 0.0001	−0.1891
C- Inoclum size	1.71	1	1.71	552.88	< 0.0001	−0.3541
AB	1.96	1	1.96	633.97	< 0.0001	0.4955
AC	0.0414	1	0.0414	13.36	0.0044	0.0719
BC	1.42	1	1.42	457.39	< 0.0001	0.4208
A²	0.3406	1	0.3406	109.97	< 0.0001	0.1537
B²	0.0402	1	0.0402	12.98	0.0048	−0.0528
C²	2.17	1	2.17	700.18	< 0.0001	0.3879
Residual	0.0310	10	0.0031
Lack of Fit	0.0232	5	0.0046	3.00	0.1264
Pure Error	0.0077	5	0.0015		Adeq Precision	72.4880
Std. Dev	0.0557		R²	0.9964
Mean	1.79		Adjusted R²	0.9931
C.V. %	3.12		Predicted R²	0.9777

ISSN: 1475-2859