Fig. 7
From: Computational analysis of protein synthesis, diffusion, and binding in compartmental biochips
Experimental validation of the 100-box model. Fluorescence signals of trap-bound gp10 synthesized in a quasi-twodimensional, silicon-based expression system recently introduced [26] (symbols) and corresponding simulation results at \(t = 21\cdot 10^3\Delta t\) (dashed lines). In the experiment, the gp10 synthesis rate is roughly proportional to the amount of gp10-coding DNA sequences. Simulations were performed in the 100-box system as described in the main text with synthesis sites being located at boxes \(i_\mathrm{syn} = 7,...,14\), binding affinity \(\kappa _\mathrm{bin}={2e-3\,.} \Delta t^{-1}\), diffusion rate \(\widetilde{D}={0.045\,.}\Delta t^{-1}\) and synthesis rate as indicated. Increasing the amount of gp10-coding DNA sequences on the biochip by a factor of 4 corresponds to an increase in the simulation parameter \(\alpha _\mathrm{syn}\) by roughly the same fold change