Table 1 Strains and plasmids used in this study
Strains | Characteristics | Source |
|---|---|---|
E. coli DH5α | Host for cloning | Lab stock |
E. coli W3110 | Wild type, starting strain | Lab stock |
Pro-1 | E. coli W3110, ∆putA | This study |
Pro-2 | Pro-1, ∆lacI, yghX::Ptrc-proB74 | This study |
Pro-3 | Pro-2, rpH::Ptrc-proB74 | This study |
Pro-4 | Pro-3, yjiT::Ptrc-proA | This study |
Pro-5 | Pro-4, yciQ::Ptrc-proC | This study |
H-1 | E. coli W3110, Ptrc-DaP4H | This study |
H-A | E. coli W3110, Ptrc99a | This study |
HYP-A | Pro-5, Ptrc99a | This study |
HYP-1 | Pro-5, Ptrc99a-DaP4H | This study |
HYP-2 | HYP-1, ∆ldhA | This study |
HYP-3 | HYP-2, ∆poxB | This study |
HYP-4 | HYP-3, ∆ackA | This study |
HYP-5 | HYP-4, ∆aceA | This study |
HYP-6-1 | HYP-5, PBBa-j23109-SucAB | This study |
HYP-6-2 | HYP-5, PBBa-j231115-SucAB | This study |
HYP-6-3 | HYP-5, PBBa-j23114-SucAB | This study |
HYP-7-1 | HYP-6-1, ycdN::Ptrc-Baxfp | This study |
HYP-7-2 | HYP-6-1, ycdN::Ptrc-Acxfp | This study |
HYP-7-3 | HYP-6-1, ycdN::Ptrc-Blxfp | This study |
HYP-8 | HYP-7-1, Ptrc-PntAB | This study |
HYP-9 | HYP-8, yjiP::Ptrc-PntAB | This study |
HYP-10 | HYP-9, yeeP::Ptrc-vgb | This study |
pGRB | gRNA expression vector | Lab stock |
pRed-cas9 | Cas9 expression vector | Lab stock |
Strains | Characteristics | Source |
E. coli DH5α | Host for cloning | Lab stock |
E. coli W3110 | Wild type, starting strain | Lab stock |
pGRB | gRNA expression vector | Lab stock |
pRed-cas9 | Cas9 expression vector | Lab stock |